A key determinant of success in the tennis serve is ball speed. Analysis of the tennis serve shows that muscle strength is only moderately associated with ball speed in college male tennis players. The relative contribution of upper and lower body strength has yet to be determined in the college female tennis player. This study investigated the relative contribution of upper body,lower body,and handgrip strength to ball speed during the tennis serve in NCAA Division I female collegiate tennis athletes. Ten players (age = 20.9 ± 1.5 yr; ht.= 164.21 ± 6.6 cm.; wt.= 61.34 ± 5.7 kg.)performed maximal isokinetic strength tests for the dominant knee extensors(300°/s)and shoulder internal rotators (300°/s)and for dominant handgrip strength. Tennis serve ball speed(kph)was measured with a radar gun over a distance of 27.4 m with the medium score of five correct trials used as datum. Means(± SD)for ball speed were 136.77 ± 11.6 kph; 80.30 ± 13.5 Nm for knee extension strength; 19.6 ± 4.1 Nm for shoulder rotation strength, and 37.60 ± 2.8 kg for handgrip strength. Regression analysis found the linear combination of strength measures were not significantly related to ball speed (F(3,9) = .59,p = .652). The multiple correlation coefficient was .41, indicating that approximately 17% of the variance of ball speed was accounted for by the linear combination of strength measures. Relative strength indices of the individual predictors showed no significant bivariate or partial correlations (Pearson r = .16 to .46; p > .05). The results imply that upper and lower body strength are only moderately associated with tennis serve ball speed. Other biomechanical and physiological parameters likely contribute to tennis serve ball speed in the college female tennis player.Keyword(s): athletics/sports, exercise/fitness, performance