Cup stacking has been adopted recently by many physical education programs. Proponents claim that cup stacking enhances hand-eye coordination, ambidexterity, quickness, and concentration. However, no empirical evidence has been published to support these claims. We examined the influence of cup stacking on hand-eye coordination and reaction time of second-grade students (n = 42; 24 boys, 18 girls). Two physical education classes were randomly assigned as either the treatment or control group and were pre- and posttested for hand-eye coordination and reaction time. The treatment group participated in a five-week cup stacking program. Repeated measures analysis of variance (ANOVA) was used to determine statistical differences at the 0.05 level. Significant improvements were noted for both hand-eye coordination and reaction time between the pre- and posttest scores for the treatment group, but not for the control group. Therefore, these results suggest that cup stacking was indeed effective at enhancing hand-eye coordination and reaction time. Keyword(s): elementary education, performance