Researchers of sport marketing indicated that the internet website could serve as a useful tool to promote athletic programs, recruit potential customers, and enhance public relations (Varki, 1996). However, perception of individuals toward effective websites of sport organizations may be affected by various factors such as gender (Rust & Varki, 1996). What are the important factors affecting perceptions of fans who often access the internet of intercollegiate athletic programs? Does the perceptual difference exist between the female and male website visitors? Conducting a study to answer these questions appears essential. The purpose of this study was to determine gender difference in perceiving website effectiveness of National Collegiate Athletic Association (NCAA) Division I athletics programs. A total of 158 (men = 84, women = 74) graduate students voluntarily took part in this study and they were trained in using the pre-defined evaluative standards. The evaluative package includes an informed consent, demographic information, and a 4-point (1 = very ineffective, 4 = very effective) Likert scale containing 10 criteria (e.g., Speed, Layout, Website Design, Availability of Links) with acceptable validity and reliability (Varki, 1996). A total of 12 websites of athletic programs in NCAA Division I were selected and evaluated by the participants using Windows 2000 of Microsoft computers. The scores of raters were separately analyzed using analyses of variance (ANOVAs) due to uncorrelated dependent variables. ANOVAs revealed significant (p < .05) mean differences between the female and male participants on the overall mean scores and dependent variables of ‘Website Design', ‘Speed', and ‘Availability of Links'. The male participants scored (Ms. = 2.80, 3.10, 2.93, and 2.52) higher than their female counterparts (Ms = 2.10, 2.33, 1.84, and 1.77). The results indicated that gender was a significant variable affecting individuals' perceptions of effectiveness of websites. Administrators of the athletic programs may adjust their home-pages to meet the needs of both genders. In order to recruit more program supporters updating functions of the websites based on the feedback of this study is suggested. Keyword(s): gender issues, sport management