The purpose of the study was to identify and examine: 1) students' sexual knowledge, sexual attitude, and their main source of sexual knowledge; 2) the differences between variables of gender, age, and self-perceived sexual knowledge in relation to sexual knowledge and sexual attitude; and 3) the relationship between sexual knowledge and sexual attitude. We noticed much disagreement in the current literature about these matters and we decided to conduct a survey to better clarify these important issues. The adapted Kinsey Institute New Report on Sex test (Reinisch & Beasley, 1990) was used to determine sexual knowledge and the Insel/Roth, Core Concepts in Health (2002) questionnaire was employed to assess students' sexual attitude. Survey method and stratified random sampling were adopted for data collection. Participants were 201 college students at a Mid-Atlantic university which consisted of 101 males and 100 females, were mainly undergraduates (94.5%), white (87.5%), between the ages of 20-22 (60%) with an age range of 17 to 46. The data were analyzed by using descriptive analyses, factorial analyses, chi-square, and Pearson correlation. The students achieved a mean score of 83% on the sexual knowledge test with all (100%) students having either an ambivalent or progressive/open-minded attitude toward sex as opposed to a traditional view of human sexuality. The top two sources of students' sexual knowledge came from friends (39.8%) and personal experience (36.3%). There were no significant differences in sexual knowledge between males and females, between younger and older students, and how students mainly obtained their sexual knowledge. Results indicated that a significant difference was found between students' self-perceived sexual knowledge and their actual sexual knowledge test scores [F (190) = 3.381, p<.05]. Students who rated themselves as having excellent sexual knowledge achieved the highest test score (M = 10.8). There was no significant difference, however, between students who rated themselves as good or average in sexual knowledge to their actual sexual knowledge test score. A significant difference was also found between male and female students in their sexual attitude [F(190) = 8.646, p<.05]. Female students (M = 43.57) were significantly more progressive/open-minded than male students (M = 40.19) in their sexual attitude. In addition, results showed a significant relationship between sexual knowledge and sexual attitude (r2=0.045, p<.05). We would like to further explore why females were more progressive/open-minded than males and why there was a significant relationship between sexual attitude and sexual knowledge.Keyword(s): health promotion, student issues, wellness/disease prevention