Americans have been facing two major social issues in recent years. Many Americans are failing to maintain the recommended daily physical activity. In addition to the alarming trends related to physical activity, people throughout the world are migrating to the US. Within the next 20 years, it is projected that one in every five children in public schools will be Latinos. Educators must address the health and wellness needs of our multicultural population. In order to provide effective educational services to students from various backgrounds, teachers' attitudes and knowledge are important. As suggested in the self-fulfilling prophecy theory, teachers' attitudes and expectations can have a direct effect on their students learning. However, there is a lack of knowledge on physical educators' attitudes and knowledge toward our multicultural population including Latino children. The primary purpose of this study was to examine physical educators' attitude and knowledge about working with Mexican Americans (MA). Secondly, this study examined the effects of multicultural training on the attitude and knowledge. Participants in this study were 91 physical educators. Attitude and knowledge levels of physical educators toward MA were measured by the Mexican American Attitude and Knowledge Scale. A one-sample t-test was employed to compare the participants' attitude and knowledge. A 2 x 2 x 2 (in-service training x pre-service training x graduate training) MANOVA, was used to examine the effects of three types of multicultural training on knowledge and attitude. One-way ANOVA were employed to explore the effects of teaching experiences. Physical educators were found to have positive attitudes and moderate knowledge levels toward MA, t=34.81, p < .01 and t=11.46, p < .01, respectively. A one-way ANOVA revealed a significant main effect for Spanish language skills on attitude, F (1, 89)=10.10, p < .01. Teachers who indicated having the ability to speak Spanish at a level that enabled them to communicate with Spanish speaking students were found to have positive attitudes toward MA than those who lacked this skill. However, this study did not support the positive effects of multicultural training. These findings indicate that physical educators have a good foundation of attitude and knowledge regard to working effectively with MA. Functional Spanish language skills appear to be a critical first step in preparing physical educators to work effectively with MA students. Also, multicultural training is shown to be lacking in specific information about working with MA.