The purpose of this study was to determine the acute effect of a single low-intensity exercise session on the lipid profiles of older women. Subjects were 14 White women women, with a mean age=67.4 ± 2.11 years, who were participants in a People with Arthritis Can Exercise (PACE) class. The study protocol required participants to refrain from exercise for 3 days before the single bout of exercise. The exercise session included range of motion activities, low-intensity resistance exercises, and cardiovascular activity from a chair and a standing position. Exercise duration was approximately 40 to 45 minutes, 10 minutes of which was cardiovascular. Mean resting heart rate was 73 ± 3 bpm, while mean peak exercise heart rate was 114 ± 5 bpm. Immediately postexercise, subjects consumed a mixed meal containing approximately 350 calories. Finger-stick blood samples were obtained to measure triglycerides, total cholesterol, HDL-C, LDL-C, and VLDL preexercise, immediate postexercise, and 2-hours postmeal. Samples were analyzed using a Cholestech LDX. A priori planned comparisons, using dependent t-tests, revealed significant differences in HDL-C and triglycerides. Examination of the means revealed that HDL-C rose after exercise (t=-5.59, p < .000) and then returned to preexercise levels postmeal (t=2.63, p < .021). Triglycerides fell postexercise (t=2.87, p < .013) and continued to fall postmeal (t=2.205, p < .046). None of the other planned comparisons was significantly different. We concluded that a single bout of low-intensity PACE-type exercises can improve triglycerides and HDL-C levels in older women. Further, it appears that low-intensity exercise moderated triglyceride levels in spite of consuming a meal immediately postexercise. In contrast, HDL-C levels returned to preexercise levels postmeal. The effects on HDL-C were either short-lived or were offset by the meal.