The purpose of this study was to examine college students’ physical activity patterns and gender differences in physical activity participation. A descriptive study was conducted. The independent variables were day of the week and gender and the dependent variable was the number of steps accumulated throughout the day. College students were recruited to wear a pedometer (Digi-Walker Model 200) for seven consecutive days at the anterior midline of the right leg during all waking hours to assess ambulatory physical activity. In addition, the students recorded their daily steps on a log-sheet. Descriptive statistics, dependent t-tests, and independent t-tests were calculated. The 31 participants in the study were 23.5 ± 3.19 years old and had an average BMI of 25.7 ± 4.29. The sample consisted of 13 (42%) females (age: 23.07 ± 3.3 years, BMI: 25.83 ± 5.09) and 18 (58%) males (age: 23.83 ± 3.16 years, BMI: 25.67 ± 3.77). Subjects averaged 9932.27 ± 2680.71 steps per day during the seven day period. Students were more active during the week than on the weekend (t=5.45, df=31, p=.0001). Females were most active on Thursday (11887 ± 4760.7 steps) with a seven day average of 9353 ± 2031.5 steps while males were most active on Friday (11854 ± 3984.8 steps) with a seven day average of 10351 ± 3053.9 steps. Daily (t=1.02, df=29, p=.31), weekday (t=0.75, df=29, p=.46), and weekend (t=1.39, df=29, p=.17) physical activity did not differ between genders. The results of this study indicate that college students were more active on weekdays than on the weekend and that ambulatory physical activity did not differ between male and female students. The results of this study are beneficial to health promotion practitioners when planning and evaluating physical activity interventions in a college population.