Significance: Youth are engaging in sexual activities that lead to numerous social and health problems, including unwed pregnancy, STDs, and dropping out of school. As a result Title V of the Social Security Act, systematic evaluations of the effect of abstinence-only sexuality programs has been able to be conducted. Purpose: The purpose of this presentation is to present the cumulative results of three years of evaluation of abstinence only programs in Maryland. Design: Based upon a series of pretests and revisions, two forms of a valid and reliable instrument were developed. These instruments were administered, on a pre-pose basis to over 400 individuals participating in after school, abstinence-only human sexuality programs. Findings: Over 400 individuals have participated in the 20 programs currently operational in Maryland. The population consisted of approximately 61% girls, and the age range was 12 – 15. In terms of knowledge, attitude, and practice scores, there were no statistically significant differences, overall, between pre and post-test scores, although some individual items did produce significant differences. Over 82% of the population indicated at the pretest they chose to remain sexually abstinent during the year prior to the study, but this dropped to 80% at the post-test. There was a 5% increase in the number of students who indicated they definitely would remain abstinent for the remainder of the year, but there was a continual increase in the percentage of students who indicated they probably would not remain abstinent until they graduated from high school or until they were married. There was a significant increase in the percentage of students who indicated, at post-test, they felt comfortable talking about sexual issues with their parents. There was a 3% increase in the number of students who indicated they were very sure they could keep to a decision not to have sex, regardless of the situation in which they found themselves. A total of 28% indicated it was OK to have sex with a serious boyfriend or girlfriend, but 63% indicated it was not OK to try to talk someone into having sex. Conclusions: In general, students exposed to abstinence-only based human sexuality programs held positive attitudes toward sexual activity prior to entering the programs, and these attitudes were reinforced. It will be important to conduct longitudinal studies to determine the long-lasting impact of these types of programs as these students progress through high school.Keyword(s): assessment, health promotion, youth-at-risk