Human sexuality courses offer college students a collection of topics that will assist them in making responsible sexual health decisions and enable them to gain a positive view of sexuality in today's culture. Current course content focuses on a holistic approach including the psychological, social, emotional, as well as the physical aspects of sexuality. Courses also address controversial topics such as sexual orientation, paraphilias, and the influence of religious beliefs on sexual behaviors. Human sexuality courses are uniquely different from general sexuality courses in that emphasis is placed on recognizing and developing lifelong social, vocational, familial, intimate, and sexual relationships. The purpose of the study was to determine the knowledge and attitudes of college students regarding their sexual lifestyle, religious beliefs, contraceptive use, abortion issues, and intimate relations. Convenience samples were taken from 413 students (107 males, 306 females) enrolled in Health Education (n = 115) and Psychology (n = 298) courses in a regional state supported university. A sixty-five item questionnaire was administered during the first two days of the Spring and Fall 2004 terms. Participants completed the survey again during the final two days of the term. Frequencies and paired sample T-test were analyzed using SPSS 12.0. Significant results were found in many of the variables. Health majors reported a significant decrease in their belief that society accepts homosexuality (p = .022). A significant increase in the acceptability of homosexuality to self increased among majors, genders, and across ethnic groups with the exception of Hispanics (p < .05). A Significant movement from heterosexual to homosexual orientation was reported among male and female Psychology students, Hispanics, Catholics, students who participated in high school sexuality courses, and students living in the suburbs (p < .05). Significant increases in levels of religiosity were reported among Health students, all females, Anglo-Americans, students living in the suburbs, Protestants, and students who participated in high school sexuality courses (p < .05). Findings suggest that the course content of the human sexuality course may influence attitudes, sexual orientation, and acceptability of sexual orientation in society as well as individual religious beliefs, having participated in a high school sexuality education course, and living in a suburban environment. Additional studies need to be conducted with the inclusion of a control group in a different discipline to determine if the college experience itself served as an influence on the related attitudes and behaviors of college students. Keyword(s): college level issues, gender issues, student issues