Significance: New compelling evidence suggests that heart disease may begin as early as two years of age with interventions needed at a younger age than previously reported. Both the Bogalusa Heart study and the Pathobiological Determinants of Atherosclerosis in Youth study reported that arterial lesions and stenosis were discovered in adolescents and young adults. The authors of the PDAY study found differences in accumulation of fatty streaks and the risk factor profiles of their participants with differences appearing as early as 15 years of age. Despite NCEP recommendations for cholesterol screening at age 20, little is known about cholesterol levels among university students and only a few studies have been conducted with no studies to date screening only sedentary participants. Design: One-hundred and eight sedentary participants who self-reported they had been inactive for the previous three months and had enrolled in a college-level health education class and were non-smokers volunteered for the study. Each participant donated approximately 20 ml of blood after fasting for 12 hours using standard venipuncture techniques in the antecubital vein in the bend of the elbow. Results: An ANOVA was calculated to reveal differences in blood lipids between females and males. The ANOVA revealed significant differences in HDL between women (61.12 mg/dL, 13.75 mg/dL) and men (46.47 mg/dL, 7.94 mg/dL, p=.0001), LDL between women (96.38 mg/dL, 29.78 mg/dL) and men (109.44 mg/dL, 31.05 mg/dL, p= .047), and TC/HDL ratio between women (2.9, .67) and men (3.8, 1.0, p= .0001). No significant differences were found in triglyceride levels (p=.806) and total cholesterol (p= .799). Percentiles (25th, 50th, 75th, 90th, and 95th) were calculated. With the exception of TC/HDL ratio all values presented below are recorded in mg/dL. Females total cholesterol percentiles were 148.75 (25th), 167 (50th), 194.25 (75th), 214.50 (90th), and 253.30 (95th). LDL percentiles were 78.75, 55.50, 109.50, 131.40, and 165.75. HDL percentiles were 53, 61, 66, 75.3, and 91.9. Triglyceride percentiles were 59.50, 76.0, 96.25, 107.30, and 172.50. Ratio of TC/HDL percentiles were 2.5, 2.7, 3.3, 3.9, and 4.37. Males total cholesterol percentiles were 150.25, 162.50, 183.75, 225.50, and 247.95. LDL percentiles were 90.50, 99, 116.50, 153.70, and 187. HDL percentiles were 40.75, 46.50, 50.50, 57.70, and 61.75. Triglyceride percentiles were 53.25, 72.50, 111.75, 123.70, and 136.55. A number of university students were identified as high risk based on lipid and triglyceride levels in this screening demonstrating the need for cholesterol screening on university campuses.Keyword(s): college level issues, disease prevention