The accumulation of 10,000 steps has been widely promoted as a daily activity goal to attain the health benefits associated with physical activity. However, there have been few large-scale studies that have examined steps per day (STEPS) in different populations. Therefore, the purpose of this study was to examine STEPS in a representative sample of college students from a large, public university in the south central United States. A cross-sectional, descriptive study was conducted with 204 males (46.3%) and 237 females (53.7%) (n = 441, age: 20.05 ± 1.82 years, BMI: 23.97 ± 3.79 m/kg2). The independent variable was gender and the dependent variable was STEPS. Participants wore the Actigraph® 7164 accelerometer, with cycle mode activated, over the iliac crest of their right hip during all waking hours for 7 consecutive days. Because there was a significant difference in STEPS among the cohorts (F [3, 437] = 7.04, p = .0001), cohort was included as a factor in statistical analyses. A two-factor ANOVA was conducted to examine STEPS by day of the week and gender. Student-Newman-Keuls post hoc tests were used to examine significant differences. Overall, students accumulated 11,473.87 ± 2,978.62 STEPS during the week. There was a significant difference in the number of STEPS by day of the week (F [6, 2867] = 25.64, p < 0.0001) with post hoc comparisons indicating that most weekdays (Monday – Friday) were different from the weekends (Saturday and Sunday). A subsequent t-test revealed the accrual of more steps on the weekdays than the weekends (t = 8.41, df = 1, p = 0.0001). When comparing STEPS between males (11,601.4 ± 2,961.2) and females (11,364.1 ± 2,995.5), there were no differences by week (F [1, 433] = 0.52, p > 0.47), by weekday (F [1, 433] = 0.76, p > 0.38), or by weekend (F [1, 427] = 0.16, p > 0.69). Our results indicate these students were exceeding the increasingly popular 10,000 STEPS recommendation and that they were more active on weekdays than on the weekend. Furthermore, the results suggest that total ambulatory activity does not differ between male and female college students.Keyword(s): health promotion, physical activity, wellness/disease prevention