Women athletes are two times as likely to sustain an ACL injury as a result of player contact and three times as likely to sustain an ACL injury through non-player contact than their male counterparts. The underlying cause of ACL injury is likely multifactorial. However, several researchers have identified knee joint laxity as a possible contributing factor. The purpose of this study was to provide a categorical comparison of knee joint laxity between male and female collegiate soccer players and male and female non-athletes. Volunteers included 39 (19 male, 20 female) apparently healthy, collegiate-level athletes from the University of Texas at El Paso’s 2001-2002 women’s intercollegiate soccer team and men’s club soccer team and 40 (20 male, 20 female) non-athletic students. All participants were tested bilaterally using the KT-1000 MEDmetric Knee Joint Ligament Arthrometer. Three tests were used to determine anterior laxity: Passive Displacement, Active Displacement, and the Lachmans test. The mean passive displacement, mean active displacement, and Lachmans (only for the left leg) were significantly lower for the athletic group than for the non-athletic group. Contrary to much of the previous research, there were no significant differences found between males and females for the passive and active drawer tests. However, females had significantly more laxity than males in the Lachmans test. Although expected gender differences were not found, the significant differences between athletes and non-athletes imply that strength and conditioning may play a more significant role in knee joint laxity than gender.